One of the features of Nuendo / Cubase is that you can process a clip or section of audio off-line with either a plug-in or built-in process. This off-line processing is non-destructive (you can undo it at any time) and allows for some very fast and flexible editing techniques.
Using the range tool select the piece of audio that you wish you tune. Use the command “Split Range” to extract the selected range into a separate event.
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Right-click and choose to process the audio with Autotune – this will pop up Autotune